Human Hematopoietic Colonies in Health and Disease
Karger Medical and Scientific Publishers, Jan 1, 2005 - Medical - 96 pages
Practical and concise, this guide addresses clinical and experimental hematologists, technicians and teachers interested in human hematopoietic stem cells. Offering a unique collection of photographs taken at steady reproducible culture conditions by a hematologist with extensive clinical and experimental experience, it fills a gap in the current hematology literature.
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Normality and abnormality in culture
Contribution of cultures to clinical hematology
accelerated phase agranulocytosis binized BM and PB BM cultures bright red CD34+ cells cell suspension CFU-Eo CFU-Eo/Ec CFU-G CFU-GEMM CFU-GM and BFU-E chronic CIMF Clinical Acta Haematol CML cP growth CMML Colonies in Health Colony contains colony growth colony-forming unit Contribution of Cultures cP growth pattern culture medium Cultures to Clinical deﬁciency diagnostic difﬁcult disease dysmorphic Enriched medium eosinophil Epo-free medium Epo-independent BFU-E erythroblasts erythroid erythroid colonies erythropoietin excess of blasts Ficoll-separated Gleevec GM-CSF granulocytes Health Fig hematological hemoglo Human Hematopoietic Colonies hypereosinophilic syndrome Increased growth Leukemic cluster growth low magniﬁcation macrophages mature May-Grünwald-Giemsa-stained normal Medium 4531 Epo-free megakaryocyte methylcellulose morphologically normal morphology myeloid colonies neutrophil granulocytes Normal BFU-E normal colonies pancytopenia patients PB cultures PB:BM ratio Picked and May-Grünwald-Giemsa-stained platelets Possible Causes pseudonormal reﬂect refractory anemia Scattered macrophages single cells resembling small CFU-G Sources of Error stem cell subcolonies thrombocytosis ture vitro