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Factors to be considered in image interpretation
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adenovirus Aebi aligned amplitude angle astigmatism biological specimens bright-field image bright-field microscopy calculated carbon support film catalase chromatic aberration corresponds Crowther crystal dark-field microscopy defocus density values determined diffraction maxima diffraction orders diffraction spots elastic electron diffraction electron micrographs electron microscopy enhancement example Fourier transform helical helix Henderson 1975 high resolution image analysis Image bar image contrast image intensity image processing image transform incident electron inelastic image inelastic scattering Klug layer line magnification maximum membrane molecules negatively-stained specimens neuraminidase numerical transform objective aperture objective lens obtained optical density optical diffraction pattern optical transform original image Ottensmeyer particle phase contrast photographic protein radiation damage reciprocal lattice reconstructed image result sample points scan scattered electrons scattering contrast shown in Fig shows spacing spatial frequencies specimen thickness subunits superposition T4 phage techniques thin specimen three-dimensional reconstruction tilt tion transfer function transmission electron microscope two-dimensional unit cell unscattered unstained zero