What people are saying - Write a review
We haven't found any reviews in the usual places.
Preparation of antigens for immunization
16 other sections not shown
acetyl-CoA carboxylase adsorbed antiserum adsorption agarose gels amount of antigen analyses antibodies antigen of interest antigen—antibody system antigenic determinants antisera antiserum ATP'ase Biochem biological fluid buffer casein centrifugation choline acetyltransferase clones complex concentration containing antibodies contaminating antigens control serum coprecipitation cytochrome b5 cytochrome oxidase detergent eluted enzyme activity enzyme or protein fatty acid synthetase ferritin ferritin conjugate glutaraldehyde immunization schedule immunoadsorbent immunoaffinity chromatography immunodiffusion immunoelectrophoresis immunoglobulin immunoisolation immunoprecipitation immunoprecipitation lines Immunotitration incubation inhibition injections iodinated isolation Mayer measured membrane antigens membrane proteins microsomal mitochondrial preparations molecular weight molecules monoamine oxidase monospecific antiserum multispecific non-immune obtained organelle particle-free supernatant plasma membrane polyacrylamide gel electrophoresis polyspecific precipitation presence of sodium problems procedures purified antibodies purified antigen pyruvate dehydrogenase rabbit radioactivity radioimmune assays radiolabelled reagents receptor sample Sepharose sodium dodecyl sulphate solubilized specific antibodies staining subunit tion tissue extract Triton trophoresis vectorial orientation vesicles volume of antiserum washed