What people are saying - Write a review
We haven't found any reviews in the usual places.
Use of protein A
Coupling of antibodies to enzymes
16 other sections not shown
0.1 M phosphate 0.1 M sodium acetic acid activity alkaline phosphatase amino groups anti anti-lg antigen antiserum assay of antigens avidin avidin-biotin Avrameas beads biotin Buffers and solutions cells Centrifuge chapter Chromatography chromogens column concentration conjugates containing coupling dase Dialyse against PBS dilution dissolve distilled water ELlSA eluted enzymatic Fab fragments filter fluorogenic fractions g/ml galactosidase glucose oxidase glutaraldehyde glycerol glycine h at room hydrogen peroxide immobilised immunoadsorbent immunoenzymatic immunoglobulins incubation labelled antibodies lgG1 litre lmmunol lncubate lysine methodology methods ml of distilled molecules monoclonal antibodies mouse NaCl nitrocellulose nitrocellulose sheet Note ONPG overnight at 4°C p-benzoquinone PBS-Tween PBS-Tween-gelatin peroxidase phosphate buffer polyclonal antibodies precipitate preparation procedure proteins purification reagents Rinse room temperature saturation sensitisation Sephadex sera serum Sigma sodium azide sodium carbonate solutions PBS specific stop the reaction supernatant Ternynck tion tissues Wash the plate