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Measurement of Total In Vivo Protein Synthesis
Preparation of Soluble Proteins
Determination of Radioactivity in Gel Slices
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_in vivo acrylamide adult animals adult rats Age of rats birth blood-brain barrier brain areas Brain of Rats brain protein synthesis Brain Soluble Proteins brain stem brain tissue buffer cannula cellulose centrifuged cerebellum cerebral cortex changes Chromatographic Pattern classes of proteins Daltons days of age days old DEAE-Cellulose Age DEAE-cellulose columns described in Methods determined Diencephalon EDTA electrophoresis fetus gradient H-amino acid mixture H-leucine half-lives hour hysterotomy ice-cold TCA intraperitoneally isotope jugular vein labelled amino acids liver molecular weight mRNA myelination NaOH neonatal one-day old animal particulate fraction particulate proteins Pattern of Rat polysome protein band protein eluted radioactive amino acids Radioactivity Profile Rat Brain Soluble rate of protein rate of synthesis reduced and run ribosomes Run on Discontinuous sample SDS polyacrylamide gels Separated on DEAE-Cellulose seven days slices Soluble and Particulate soluble fraction soluble in ice-cold Soluble Proteins Separated specific radioactivity staining patterns studies supernatant tubes vitro