Induction and Analysis of Antigen-Specific T Cell Responses in Melonoma Patients and Animal Models
Annotation. In the last century, it has become clear that in patients suffering certain types of cancer, spontaneous activation of the immune sytem may lead to curation. The concept of immunotherapy is based on that notion. The intention is to stimulate T cells directed against the cancer, leading to the eradication of cancer cells. However, the methods to stimulate T cell immunity are just evolving, and at the moment they are functional in small laboratory animals only. Furthermore, we know now that many cancer cells possess the capacity to scive attacks of the immune system. This thesis introduces a novel T cell vaccination method that uses a tattoo machine to inject DNA in the skin of the vaccinee. In comparison to other experimental vaccination methods DNA tattooing is very strong: besides small laboratry animals also large animals (mockeys) mount strong T cell responses on tattoo DNA vaccination. Future tests in melanoma patients will point out whether DNA tattoo vaccination is equally efficient in humans, and whether it may have a therapeutic effect in these patients. This title can be previewed in Google Books - http://books.google.com/books?vid=ISBN9789087280116.
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Acknowledgements and Curriculum Vitae
adjuvants analysis animals antibodies antigen expression antigen presentation antigen stability antigen-specific T cell APCs apoptosis CD8+ T cell cell epitopes cell responses cells expressing cellular clinical complexes cross-presentation cytokine cytotoxic T cell danger signals detected DNA tattooing DNA vaccination ELIspot emission encoding epitope flow cytometry fluorescent fusion proteins gene GILGFVFJL half-life immunization methods immunogenicity Immunol immunotherapy influenza intradermal intradermal DNA vaccination intramuscular DNA intravital imaging ligands luciferase luciferase activity lucNP lymph node lymphocytes MART-1-specific T cells melanoma patients melanoma-specific T cells MHC class MHC exchange tetramers MHC molecules MHC tetramer mouse Netherlands Cancer Institute NP366-specific NYVAC pathogen PBMC peptide vaccination peripheral blood phenotype plasmid post vaccination Proc Natl Acad proteasomal regimen samples short-interval skin specific T cells T-cell tattoo DNA vaccination tattoo vaccination tested tetramer staining transfected transgenic tumor antigens tumor specific Ub-M-lucNP ulcNP vaccine-encoded antigens variants vector viral virus vivo