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MATERIALS AND METHODS
KINETICS OF PROTEIN SYNTHESIS IN SELECTED PICORNA
3 other sections not shown
15S particle 30 minutes 40 mM magnesium abortive inactivation actinomycin agar overlay amino acids Biol bovine serum albumin buffer capsid proteins cardioviruses cell extracts cell monolayers cells infected counts cytoplasmic extracts daltons described in Chapter described in Figure determined Earle's saline electropherograms eluted EMC polypeptides EMC virions EMC virus EMC-infected cells EMC-infected HeLa cells enterovirus experiments extracts from EMC-infected glycerol glycerol gradient guanidine H-leucine labeled host protein synthesis infected cells inhibited Jacobson and Baltimore labeled EMC labeled virus leucine magnesium chloride Maizel marker mature virions ME-virus Medium A containing mengovirus minutes at room ml of PBSA molecular weight peak peptides picornaviruses plaque assay plaque-forming plates polio poliovirus poliovirus type polyacrylamide gels PPOT presence of guanidine procapsids procedure protein synthesis purified radioactivity ribonuclease room temperature Rueckert scintillation solvent shown in Figure sodium sodium hydroxide stable polypeptides strain subunits superinfected viral protein synthesis virions Virology virus attachment virus-specific viruses washed