Introduction to Flow Cytometry

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Cambridge University Press, Nov 11, 2004 - Medical - 443 pages
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Flow cytometry is a technique for measuring both scattered light and fluorescence from single cells at very rapid rates. Typically up to 5000 cells can be analysed per second. Using various fluorochromes this allows a cell population to be analysed for cells showing certain characteristics such as the presence of a particular enzyme, cellular constituent or other gene product. The information it can provide is invaluable in helping to diagnose certain cancers as well as aiding pure research into many aspects of cell biology such as the cell cycle and gene expression. This book describes the technology in a simple and direct way. The fundamental concepts upon which the technology is based are outlined and the book goes on to describe flow cytometers and what can be gained from using them in biology and medicine. The book gives a particularly detailed account of how artifactual results can arise and where 'noise' is generated. Anyone starting to use, or already using this technique, will need to read this book.
 

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Contents

1 Introduction
1
2 Fluid flow dynamics
5
3 Light and optics
18
4 Electronics
65
5 Computing
74
6 Cell sorting
106
7 Preparation and staining
117
8 Miscellaneous techniques
137
11 Nucleic acid analysis
201
12 Nucleic acids and protein
266
13 Chromosomes
288
14 Dynamic cellular events
309
15 Applications in oncology
345
16 Epilogue
385
References
387
Index
431

9 Instrument performance
150
10 Light scatter applications
186

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