Introduction to fluorescence microscopy
This handbook provides a thorough and detailed introduction to fluorescence microscopy by exploring three main areas of the field. First discussed are the physical principles of the phenomenon of fluorescence and fluorescence microscopy, including transmitted versus incident illumination. Next is a detailed description of various microscope parts, including lenses, filters, and light sources, and a discussion of the main applications of fluorescence microscopy, including immunofluorescence, microflurometry, and photomicrography. The final section enables the reader to solve the most common problems in fluorescence microscopy. Each chapter includes an up-to-date list of review articles and books for further reading, and there are illustrations throughout the text of typical microscopes and optical components. Biologists, students, and researchers of fluorescence microscopy will value this clear and indispensable guide to the field.
What people are saying - Write a review
We haven't found any reviews in the usual places.
Introduction to fluorescence
Practical fluorescence microscopy
3 other sections not shown
A(nm absorbance absorption antibody antigen apochromat autofluorescence background band-pass filter barrier filter blue bright field condenser calibration camera cells chromatic beam splitter chrome colour components conjugate dark ground condenser detection unit electronic emission spectra emitted fluorescence energy epi-illumination escence Ethanol example excitation and emission excitation filter exposure eyepiece fading field diaphragm films FITC FITC and TRITC flow cytometry fluorescence intensity fluorescence light fluorescence measurements fluorescence micro fluorescence microscopy fluorescing images fluorescing objects fluoro fluorochrome fluorometers fluorophore focused given in Fig glass high numerical aperture immunofluorescence incident illumination interference filters Koehler illumination labelling lamp-housing lens lenses light intensity microfluorometry mirror molecules non-specific fluorescence numerical aperture observed obtained optical pass filter photodetector photomicrography photomultiplier Ploem quantitative FM quantum efficiency radiation reaction result scanners scanning selection sensitive shown in Fig specific specimen standard Transmission characteristics transmitted illumination transmitted light illumination unwanted excitation light wavelength xenon lamps