Introduction to Plant Biotechnology (3/e)
This book has been written to meet the needs of students for biotechnology courses at various levels of undergraduate and graduate studies. This book covers all the important aspects of plant tissue culture viz. nutrition media, micropropagation, organ culture, cell suspension culture, haploid culture, protoplast isolation and fusion, secondary metabolite production, somaclonal variation and cryopreservation. For good understanding of recombinant DNA technology, chapters on genetic material, organization of DNA in the genome and basic techniques involved in recombinant DNA technology have been added. Different aspects on rDNA technology covered gene cloning, isolation of plant genes, transposons and gene tagging, in vitro mutagenesis, PCR, molecular markers and marker assisted selection, gene transfer methods, chloroplast and mitochondrion DNA transformation, genomics and bioinformatics. Genomics covers functional and structural genomics, proteomics, metabolomics, sequencing status of different organisms and DNA chip technology. Application of biotechnology has been discussed as transgenics in crop improvement and impact of recombinant DNA technology mainly in relation to biotech crops.
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activity Agrobacterium amino acid amplified analysis anther antibody antisense Arabidopsis auxin bacterial biosafety biotech biotechnology callus cDNA chloroplast chromosome cloning coding coli containing crops cytokinin databases detection DNA fragments DNA molecules DNA polymerase DNA sequence electrophoresis embryos encoding enzyme etal eukaryotic explant expression function fusion gene transfer genetic engineering genome GMOs growth haploid herbicide herbicide tolerant insertion isolated maize mapping marker gene medium membrane method molecular mRNA mutations nucleotide oligonucleotide organisms patent pathogen phage phenotype plant cells plant tissue plant tissue culture plasmid pollen polymorphism potato primer probe production promoter protein protoplasts reaction recombinant regeneration region replication resistance gene restriction result retrotransposons RFLP rice seed selectable marker single somatic hybrids soybean species sterile strand structure synthesis T-DNA target technique tissue culture tomato toxic traits transcription transformation transgenic plants transgenic tobacco transposon variety vector viral virus viruses vitro yeast