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Detection and Enumeration ofT Cells in Tissues
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agarose analysis antigen assay BCGF cell growth factor cell lines cell populations cell subsets cell suspension cell-mediated immunity cells/ml cellular centrifugation chemotactic Clinical Immunology clones concentration conjugates cutaneous cytotoxic delayed hypersensitivity dilution disease effector cells Experimental Medicine Ficoll-Hypaque flow cytometry fluorescence Geczy gradient HBSS helper helper cells Herberman R B human T cell hybridoma Immunology 129 incubation induced infection interleukin-2 Journal of Experimental Journal of Immunology laboratory leukocytes lymphoid cells lymphokines lymphotoxin macrophages medium mitogen molecules monoclonal antibodies monocytes mononuclear cells murine natural killer cells NK cells normal number of cells patients peripheral blood plasma plates production proliferation reaction reactivity Reinherz E L response resuspended room temperature RPMI Schlossman S F sensitive serum skin test solution specific staining sterile stimulation studies subpopulations supernatant suppressor suppressor T cells T4/T8 ratio T4+ cells target cells technique test antigens thymocytes tubes tumor cells vitro vivo washed