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Detection and Enumeration of T Cells in Tissues
7 other sections not shown
agarose analysis antigen assay BCGF cell activation cell growth factor cell lines cell populations cell subsets cell suspension cell-mediated immunity cellular centrifugation chemotactic cloned concentration conjugates cytotoxic delayed hypersensitivity dilution disease effector cells Experimental Medicine Ficoll-Hypaque flow cytometry fluorescence Geczy gradient HBSS helper helper cells Herberman R B human T cell hybridoma immunodeficiency Immunology 129 incubation induced infection interleukin-2 Journal of Experimental Journal of Immunology laboratory leukocytes lymphoid cells lymphokines lymphotoxin macrophages medium mitogen molecules monoclonal antibodies monocytes mononuclear cells murine natural killer cells NK cells normal number of cells patients peripheral blood plasma plates production proliferation reactions reactivity Reinherz E L response resuspended room temperature RPMI Schlossman SF sensitive serum skin test specific staining sterile stimulation studies subpopulations supernatant suppressor suppressor T cells T4/T8 ratio target cells TCGF receptor technique test antigens thymocytes tubes tumor cells vitro vivo washed