Lab Manual in Biochemistry: Immunology and Biotechnology
"It is a complete instruction manual that imparts knowledge on principles, protocols and applications on techniques of biochemistry, immunology and biotechnology accurately in a user-friendly style."
Prof R C KuhadHead, Department of MicrobiologyUniversity of Delhi South Campus
Lab Manual is intended to be a handy reference for undergraduate and postgraduate students in life science and allied fields. The book covers fundamental exercises as well as advanced protocols, along with authentic explanation of various techniques and precautions pertaining to common errors in the laboratory.
Divided into 10 sections, Lab Manual covers:
1.Techniques of biochemistry and clinical biochemistry, cell biology enzymology
2.Latest techniques of immunology
3.Isolation and cultivation of microbial and eukaryotic viruses, methods of direct gene transfer, mutagenesis and recombinant DNA technology.
Written by authors who have over a decade of experience in teaching and research, this book will serve the needs of:
*Practitioners in medical and research labs of applied biological sciences.
*Professionals from interlinked disciplines such as human nutrition, medical instrumentation, etc.
*Students of biochemistry, microbiology, immunology, genetics and biotechnology.
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absorbance acetic acid acrylamide agar alkaline alkaline phosphatase amino acids amylase antibodies antigen assay bacterial bilirubin biomolecules blank blood blot buffer of pH Calculate cells cellulose Centrifuge cholesterol chromatography chromosomes coli color column commercially available conc concentration conjugate containing contents denatured detect dialysis diluted dissolving EDTA electrophoresis ELISA eluted enzyme enzyme activity estimate ethanol fatty acids fractions gene genome glass glucose glucose oxidase heat hemoglobin Incubate ions lipids membrane method ml distilled water molecular weight molecules mutagen NaOH ninhydrin nitrocellulose osazone pellet phage phase phenol phosphatase phosphate buffer plasmid plasmid DNA plate precipitate present PRINCIPLE PROCEDURE produced protein reaction mixture reagent refer to experiment room temperature salt SDS-PAGE separate serum sodium soluble solvent specific spectrophotometer stain standard curve sterile stock solution substrate sulfate supernatant technique test tube tion titration transfer Tris-HCl urine sample vector vitamin volume wash