Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench
Work at the biology bench requires an ever-increasing knowledge of mathematical methods and formulae. In Lab Math,Dany Spencer Adams has compiled the most common mathematical concepts and methods in molecular biology, and provided clear, straightforward guidance on their application to research investigations. Subjects range from basics such as scientific notation and measuring and making solutions, to more complex activities like quantifying and designing nucleic acids and analyzing protein activity. Tips on how to present mathematical data and statistical analysis are included. A reference section features useful tables, conversion charts and ‚€œplug and chug‚€ equations for experimental procedures. This volume is an excellent, structured source of information that in many laboratories is often scattered and informally organized.
Chemistry by the Numbers
Equipment for Measuring Counting and Otherwise
DNA and RNA
Collecting Interpreting and Presenting
Reference Tables and Equations
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absorbance acceptor Alexa Fluor amino acid amount atom axis base Bound to DNA Bound to protein buffer calculate calibrate centration centrifuge Chapter chemical concentration constant container conversion factors convert count cuvette described descriptive statistics determine dilution dimensions dsDNA electrons equation estimate example extinction coefficient final volume fluorescence fluorophores fragment FRET grams graph hemocytometer Hoechst kilograms labeled length light liter mass measure method mg/ml molarity mole molecular weight molecules multiply normally distributed nucleic acids nucleotides number of cells Number of G objective ocular micrometer oligonucleotide p-value particles pH meter pipette pipette tip pmole population precision protein protons quantify radioactivity ratio reactants reaction rate relationship residues scientific notation significant digits solvent spectrophotometer standard curve standard deviation stock solution symbol tell temperature tion total number unit fractions units variables voltage wavelength zero
Page 61 - As far as the laws of mathematics refer to reality, they are not certain; and as far as they are certain, they do not refer to reality.
Page 30 - The candela is the luminous intensity, in a given direction, of a source that emits monochromatic radiation of frequency 540 x 1012 hertz and that has a radiant intensity in that direction of (1/683) watt per steradian.
Page 29 - The ampere is that constant current which, if maintained in two straight parallel conductors of infinite length, of negligible circular cross-section, and placed 1 meter apart in vacuum, would produce between these conductors a force equal to 2 x 10"7 newton per meter of length (9th CGPM, 1948).
Page 98 - Avogadro's law states that equal volumes of all gases at the same temperature and pressure contain the same number of molecules...
Page 24 - When the mole is used, the elementary entities must be specified and may be atoms, molecules, ions, electrons, other particles, or specified groups of such particles.
Page 27 - The kelvin, unit of thermodynamic temperature, is the fraction 1/273.16 of the thermodynamic temperature of the triple point of water (13th CGPM, 1967).
Page 29 - It may be added here that, if the direction of the current is from a point of higher potential to a point of lower potential...
Page 42 - The proton is a positively charged particle located in the nucleus of an atom. The number of protons in the nucleus of an atom determines the atomic number of that element.
Page 196 - Now having (I know not by what accident) engaged my thoughts upon the Bills of Mortality, and so far succeed-||ed therein, as to have reduced several great confused Volumes into a few perspicuous Tables, and abridged such Observations as naturally flowed from them, into a few succincT; Paragraphs, without any long Series of multiloquious Deductions...
Page 44 - O On the right of the periodic table are the noble gases. These elements have full outer shells. O Elements in the same group have the same number of electrons in their outer shells. O The number of electrons O The transition metals are in orbiting the nucleus increases the middle of the table, down each group.