Laboratory Exercises in Developmental Biology

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Academic Press, Dec 2, 2012 - Science - 241 pages
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This intensive manual provides students with valuable information and insights into animal development at the organismal, cellular, and subcellular levels. The book uses both descriptive and investigative approaches that emphasize techniques, key experiments, and data analysis.
  • Provides a broad introductory view of developmental systems
  • Teaches both classical embryology and modern experimental approaches
  • Contains seventeen laboratory exercises, written in step-by-step style
  • Organized with additional notes to students and preparators
  • Lists questions and references for each exercise
  • Special chapters give introductions to the scientific process, use of the microscope, and the writing of scientific papers
  • Illustrated with detailed line drawings
 

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Contents

EXERCISE 1 Fertilization in the Sea Urchin
11
EXERCISE 2 Early Development in the Sea Urchin and Starfish
23
To Neurulation
35
To 10 Millimeters
49
EXERCISE 5 Preventing Axis Formation in Amphibians with Ultraviolet Irradiation
65
To 48 Hours
81
To 96 Hours
97
EXERCISE 8 Chick Development in a Windowed Egg
111
EXERCISE 11 Cardia Bifida in the Chick
147
EXERCISE 12 Tadpoles and Thyroxine
161
EXERCISE 13 Blastomere Totipotency in the TwoCell Mouse Embryo
175
EXERCISE 14 Alizarin Staining
189
EXERCISE 15 Drosophila Polytene Chromosomes
199
EXERCISE 16 Induction of Chromosome Puffing with Heat Shock
213
EXERCISE 17 Drosophila Imaginal Discs and Ecdysone
223
INDEX
235

EXERCISE 9 Ethyl Alcohol Caffeine and Chick Development
123
EXERCISE 10 Totipotency of the Unincubated Chick Blastoderm
137

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