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Cooperativity between the Catalytic Sites
REGULATION OF ASPARTATE
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5-mM CAP 50-mM Tris-acetate absorption acrylamide affinity allosteric amino acids amount aspartate assay ATCase ATCase activity ATCase operon ATP and CTP calculate calibration carbamylphosphate catalytic and regulatory catalytic chains catalytic sites cell centrifugation column constant containing cooperative denatured determine dimer dissociation effectors elution Enzyme solution equation exclusion chromatography Experimental Conditions Figure fractions function incubation inhibition inhibitor interactions isolated catalytic subunits ligand macromolecule Maire measured method mg/ml migration mixture molar mass molecular mass molecules native ATCase obtained operon optical density ovalbumin parameters phosphate buffer pH plot pores presence previously described protein bands radioactive rate of reaction reagent regulatory chains regulatory subunits Results Fig sample saturation curve sedimentation coefficient sedimentation equilibrium Sepharose size exclusion chromatography Stokes radius structure substrate substrate binding succinate temperature tion Tris-acetate uracil values