Laboratory Manual in General Microbiology

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Wiley, 1916 - Bacteriology - 422 pages
 

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Page 12 - Water, syringes, surgical dressings, bedding, india-rubber apparatus, filters, old. cultivations, culture media, etc., not injured by high ternSTERILIZATION peratures, may be more quickly sterilized by heating in steam under pressure. Exposure to steam at a temperature of 115° C. for twenty minutes is in most cases sufficient to insure sterilization, FIG. 4. — Autoclav, Horizontal, for Steam or Gas. FIG. 5.— Autoclav, Vertical, for Gas Only. but some media, potato for instance, require a temperature...
Page 189 - ... milk in recent years. It remains to be shown to what extent these enzymes are secreted by the cow and how far they are elaborated by the bacteria which chance to be dwelling within the particular udder.
Page 378 - Measure. 10 millimeters 1 centimeter, 10 centimeters 1 decimeter, 10 decimeters 1 meter, 10 meters 1 dekameter, 10 dekameters 1 hektometer, 10 hektometers 1 kilometer. RELATIVE VALUE OF APOTHECARIES' AND IMPERIAL MEASURE. APOTHECARIES'.
Page 380 - WEIGHT. 10 milligrams = 1 centigram. 10 centigrams = 1 decigram. 10 decigrams = 1 gram. 10 grams = 1 decagram. 10 decagrams = 1 hectogram. 10 hectograms = 1 kilogram.
Page 312 - Infection, Immunity and Specific Therapy (1915), pp. 68, 69, 79, 152, 266-291. MARSHALL: Microbiology (1911), pp. 488, 567-570. MCFARLAND: Pathogenic Bacteria and. Protozoa, 7th Ed. (1912), pp. 149-152. GILTNER: Studies of Agglutination Reactions in Hog Cholera during the Process of Serum Production (Preliminary) Tech. Bul. 3 ' (1909), Mich. Agr. Expt. Sta.
Page 365 - Mercuric chloride is exceedingly corrosive as is also the acid in which it is originally dissolved; therefore it should not be placed in metal containers or agateware pails, cups, etc., if the enamel is chipped sufficiently to expose the metal. Remember that mercuric chloride is a DEADLY POISON ! Great care must be exercised in properly labelling all bottles, etc., containing it. Phenol: (CeHsOH) long colorless crystals that become pink upon exposure to light and air. Synonyms: Carbolic acid, phenic...
Page 315 - Ib. pig. Make daily observations of the pig and record the temperature each day. 5. When undoubted symptoms of hog cholera have developed, kill the pig and make a careful autopsy. Save the blocd in a sterile jar. 6. Repeat the experiment, using blood procured in 4 as virus. 7. By repeated nltrations and injecting into susceptible hogs, it may be proven that a living microorganism, incapable of producing visible growth in vitro, passes through the filter and develops in the body of the Pig8. State...
Page 183 - CH3COOH If there is not plenty of air present the oxidation may not become complete and small amounts of acetaldehyde may form, ie, the reaction stops at the first stage. If the initial percentage of alcohol is below 1 to 2% the vinegar bacteria will soon attack the acetic acid, oxidizing it completely to carbon dioxide and water, as follows...
Page 200 - Examine both sets of cultures at the end of twentyfour hours and forty-eight hours for growth. 8. Compare the three sets of cultures and note the variations from the normal type of growth. Tabulate your data. 9. Are all of these organisms pecilothermic? What are termed the cardinal points of temperature for microorganisms? What is the lowest temperature at which growth, even of the feeblest kind, is possible? What term is applied to organisms which grow best at low temperatures? 10. Give all results...
Page 181 - Ammonium carbonate: mease — specific term. III. Oxidizing enzymes : oxidases — general term, of : A. Ethyl alcohol: alcoholase (alcoholoxidase, vinegaroxidase) — specific term. B. Organic acids: 1. Lactic acid: lactacidase — specific term. 2. Acetic acid: acetacidase — specific term. C. Tyrosin: tyrosinase — specific term. IV. Reducing enzymes: reductases — general term, of: A. Hydrogen peroxide : 1. Catalase — specific term, free oxygen liberated. 2. Peroxidase — specific term,...

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