Laboratory Methods in Special Medical Bacteriology: Course #8390-C : TechniquesU.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, 1986 - Bacteriology - 49 pages |
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125 mm tubes 15 minutes 24-hour infusion broth according to commercial acetamide acetamide agar acid Adjust pH Adjust to pH alkaline Autoclave Autoclave 15 Autoclave at 121 Basal medium biochemical blood agar plate carbohydrate cells Cetrimide commercial directions Difco Dissolve Distilled water drop esculin Ethanol final concentration flagella flagella stain glucose gm 5 gm Gram Negative Gram stains growth heart infusion agar heart infusion broth HIA slant hydrolysis Incubate at 35-37 infusion broth culture Inoculate lecithinase loopful lysis ml amounts ml Distilled water ml in 15 ml per tube Moraxella osloensis MR-VP broth NaCl nitrate broth nonfermenting nutrient broth Observe daily organisms overnight oxidase oxidation peptone pigments Prepare Pseudomonas aeruginosa reagent room temperature safranin sheep blood Simmon's slide smear solution spores sterile streak Sudan black suspension Test procedure test reagents TGY slant tryptone Tube 5 ml Tube in 15 urea agar xylene zone