Lectin Histochemistry: A Concise Practical Handbook
A succinct and highly practical guide to lectin histochemistry. It is aimed at laboratory-based scientists with little or no experience of working with lectins.
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40 other sections not shown
3x in lectin acid affinity chromatography agarose agglutination agglutinin alkaline phosphatase antibodies aqueous mountant avidin beads biotin block blood group blotting carbohydrate carbohydrate residues carbohydrate structures cell or tissue Chromogenic substrate column complex concentration counterstain Dehydrate detection dialysis distilled water endogenous peroxidase enzyme enzyme labels ethanol example filter paper fluorescent labels galNAc given in Box glcNAc glu glu glycoconjugates glycolipids glycoproteins glycosaminoglycans glycosylation haemagglutination haematoxylin Histochem histochemistry horseradish peroxidase human illustrated in Figure Incubate inhibition labelled lectin lectin binding lectin buffer lectin histochemistry ligand membrane method microscope slide ml distilled water molecular weight monosaccharide mounted neoglycoprotein nitrocellulose oligosaccharides paraffin sections peroxide poly-L-lysine polysaccharides precipitate protein resinous mountant room temperature running tap water sample SDS-PAGE serum smears sodium specific staining stock solution streptavidin sugar binding suitable techniques tissue preparation tissue sections trypsinization tubing UEA-I ug ml-1 Wash in running water for approximately