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Preparation of Sections
Stains for Connective Tissue
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24 hours 30 minutes absolute alcohol absolute and 95 acid alcohol alcohols to distilled alum ammonia water Aniline blue Basic fuchsin block BLUE SOLUTION celloidin changes of 95 changes of absolute changes of xylene chloroform Collagen concentrated 1.0 cc Counterstain Cut paraffin sections Cut sections decalcifying Dehydrate Deparaffinize Differentiate Dissolve Distilled water 100.0 eosin FAST RED KERNECHTROT fibers fluid formalin frozen sections fuchsin solution gelatin Glacial acetic acid Harris's hematoxylin hematoxylin solution Hydrochloric acid iodine knife lithium carbonate Mallory microns microscope mount in Permount nuclear fast red Nuclei Pathological Technique Permount or balsam Phosphomolybdic acid Phosphotungstic acid Picric acid POTASSIUM FERROCYANIDE REFERENCE Remove mercury precipitates RESULTS Rinse in distilled running tap water running water silver nitrate SILVER NITRATE SOLUTION slide sodium thiosulfate Stain FIXATION STAINING PROCEDURE stock solution tap water W. B. Saunders Wash in distilled Wash in running Wash in tap Wash in water Weigert's WEIGERT'S IRON HEMATOXYLIN