Marrow Stromal Cell Culture
Jon N. Beresford, Maureen E. Owen
Cambridge University Press, Apr 16, 1998 - Medical - 153 pages
The first book of its kind on marrow stromal cells, and written by leading experts in the field, this concise handbook aims to provide all that is needed to those involved in this relatively young area of research. The book provides a historical perspective and a summary of present knowledge of the marrow stromal lineages that are currently best understood. The contributors provide detailed protocols for the isolation, culture, and characterization of marrow stromal cell types from human and other animal species. The breadth and depth of the subject matter included makes the book equally suitable for new researchers and the more experienced investigator in this rapidly expanding field.
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1 The marrow stromal cell system
ontogeny structure cellular composition and changes in disease
3 Isolation purification and in vitro manipulation of human bone marrow stromal precursor cells ...
4 Isolation and culture of human bonederived cells
5 Marrow stromal adipocytes
Other editions - View all
adipocytes adipogenesis adipogenic adipose adult alkaline phosphatase antigen articular ascorbic acid assay Aubin Beresford Bianco bone marrow bone marrow stromal bone sialoprotein bone-derived cells bovine Cancedda cartilage cell culture cell populations cell types cellular centrifugation CFU-F chondrocytes cloning collagen type collagenase colonies conﬂuence containing culture medium cytokines derived dexamethasone digestion dilution endothelial cells explants expression extracellular matrix fetal ﬁbroblasts ﬁnal ﬁrst ﬂask ﬂow formation Friedenstein Gimble glucocorticoid Gronthos growth factor growth medium Gundle haematopoietic cells HBDC human bone identiﬁed incubated inﬂuence L-ascorbic marrow adipocytes marrow cavity marrow stromal cells mature mineralised minutes monoclonal antibody morphology mRNA osteoblast osteoblast lineage osteocalcin osteocytes osteogenic differentiation osteoprogenitor pericytes Petri dish phenotype preadipocytes presence primary culture progenitor cells proliferation protein receptor resuspended reticular cells retinas RMSC Schor serum Sigma Simmons solution speciﬁc stages staining stromal stem cells studies synthesis tion tissue trypsin vessels vitro washed