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CHAPTER II Factors Underlying Significant Underestimations
CHAPTER III The use of 23H glucose to assess rates
CHAPTER IV Measurements of glucose phosphorylation in intact
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2.5 mM dithioerythritol 3H2O formation activation of glucokinase Alvares and Nordlie Arion assay for glucokinase assay medium Biochem Biol blood glucose buffer catalyzed Chem concentration of glucose-6-P continuous assay decrease diabetic digitonin dilution discontinuous assay dithioerythritol enzyme estimated factors fasted rats fed rats fructose-6-P futile cycling glucagon glucokinase activity gluconeogenesis gluconeogenic precursors glucose and gluconeogenic glucose concentrations glucose isomerase glucose phosphorylation glucose production glucose uptake glucose-6 glucose-6-P glucose-6-P concentration glucose-6-phosphatase glucose-6-phosphatase activity glycogen synthesis hepatic glucose output hepatocytes isolated hexokinase hexose homogenates hydrolysis increase incubated inhibition insulin intact hepatocytes isolated from fasted isolated hepatocytes kinase labeled lactate dehydrogenase liver liver extracts Marden metabolite microsomal Newgard Nordlie perfused phosphate phosphoglucose isomerase postmicrosomal supernatants present Progress curves rat liver rate of 3H2O rate of detritiation rate of formation rate of glucose sonication specific activity substrate supernatants tion tritium underestimation units/g vitro vivo VMAX2 VMAX3 XPGL