Mechanisms and Regulation of Papovavirus DNA Replication

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ProQuest, 2006 - 278 pages
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Papovaviruses are a group of small DNA viruses that infect animals and can potentially cause tumors in both the animals they infect and cell culture systems. Due to the relative simplicity of their genomes, papovaviruses rely upon the host replication machinery to facilitate their replication. Since only one or two viral gene products are required for replication of the viral genome, papovaviruses behave at the biochemical level in a manner very similar to the host cell. Papovaviruses are one of the most easily manipulated and well-established models known to study mechanisms of various viral and host eukaryotic systems. Using both human papillomavirus type 11 (HPV-11) and the polyomavirus simian virus 40 (SV40), several mechanisms of DNA replication regulation were investigated. The interaction between two replication proteins that are essential during HPV-11 replication are determined. Then, the effect of an inhibitory human transcription factor on HPV replication is presented. Finally, using the well-established SV40 in vitro replication assay, the poorly understood DNA damage induced intra-S phase checkpoint is further characterized. These studies together, using papovaviruses as powerful models, will extend our knowledge of the processes by which both viruses and their human hosts regulate the processes involved in DNA replication and repair, which will also further the field in the context of cancer progression in humans.
 

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Contents

Chapter II
19
Chapter III
39
Chapter IV
76
respective kinases
118
Active ATR is recruited to SV40 replicons in the absence
134
Chapter V
142
Figure M1 Chromatographic separation of 293 extract
154
Preliminary findings and future directions
155
Chapter VI
181
The two major Sphase checkpoint pathways of ATMATR signaling
184
Phosphorylation of papovavirus replication proteins by purified
197
Phosphorylation of RPA during SV40 DNA replication
210
An overall model for ATR mediated inhibition of in vitro
225
Appendix
227
checkpoint response
228
Copyright

Mixing of MO59J extract with checkpoint competent 293 extract
170

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