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acid aerobic agglutination alcohol amount anaerobic animals antibiotic antibody antigen antiserum autoclave bacilli bacteria Bacteriology blood agar bottles broth buffer cent centrifuge chloride citrate clinical coli coliform colonies colour concentration containing cotton-wool coverslip diameter dilution disease disinfectant disks Dissolve distilled water examined faeces fermentation film filter fluid gelatin glass glucose Gram-negative growth guinea-pig haemolytic heat identification incubation infection inhibition inoculation inoculum isolation Journal Laboratory Diagnosis lactose liquid medium membrane method methylene blue Microbiology microscope milk mixed mixture motile mycoplasma nutrient agar organisms pathogenic Pathology penicillin peptone Petri dish phenol phosphate pipette plate positive prepared produce rabbit reaction reagent red cells removed saline salmonellae sensitive sera serological serotypes serum shigellae slide sodium Sodium chloride solution species specimens spirochaetes spores stain staphylococci steam sterile subculture suitable surface suspension swabs syringe temperature tion tissue titre toxin tube virus viruses volume washed welchii