Metabolomics in Practice: Successful Strategies to Generate and Analyze Metabolic DataMichael Lämmerhofer, Wolfram Weckwerth Unlike other handbooks in this emerging field, this guide focuses on the challenges and critical parameters in running a metabolomics study, including such often-neglected issues as sample preparation, choice of separation and detection method, recording and evaluating data as well as method validation. By systematically covering the entire workflow, from sample preparation to data processing, the insight and advice offered here helps to clear the hurdles in setting up and running a successful analysis, resulting in high-quality data from every experiment. Based on more than a decade of practical experience in developing, optimizing and validating metabolomics approaches as a routine technology in the academic and industrial research laboratory, the lessons taught here are highly relevant for all systems-level approaches, whether in systems biology, biotechnology, toxicology or pharmaceutical sciences. From the Contents: * Sampling and Sample Preparation in Microbial Metabolomics * Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics * GC-MS, LC-MS, CE-MS and Ultrahigh Resolution MS (FTICR-MS) in Metabolomics * NMR-based metabolomics analysis * Potential of Microfluidics and Single Cell Analysis in Metabolomics * Data Processing in Metabolomics * Validation and Measurement Uncertainty in Metabolomic Studies * Metabolomics and its Role in the Study of Mammalian Systems and in Plant Sciences * Metabolomics in Biotechnology and Nutritional Metabolomics and more. |
Contents
Tandem Mass S ectrometr H henated with | |
References | |
The Potential of Ultrahigh Resolution MS lFTlCR | |
Gas Chromatography and Comprehensive Two | |
LCMSBased Nontargeted Metabolomics | |
The Art and Practice of Lipidomics | |
Metabolomics Application in Plant Sciences | |
The Role of CEMS in Metabolomics | |
NMRBased Metabolomics Analysis | |
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Metabolomics in Practice: Successful Strategies to Generate and Analyze ... Michael Lämmerhofer,Wolfram Weckwerth No preview available - 2013 |
Common terms and phrases
amino acids ammonium ammonium acetate Anal analytical anionic applied approach Biochem Biol biological samples biomarkers Biotechnol blood plasma capillary carbon CE-MS cell Chem chemical Choi Chromatogr column combined complex compounds concentration correlation cross-validation data analysis data processing databases deconvolution derivatization detection developed Electrophoresis electrospray ionization elution example extraction fatty acid Fiehn Figure flux analysis formic acid GC-MS injection instrument internal standard intracellular ionization mode isotope isotopically labeled isotopomer labeling LC-MS levels lipid lipid classes lipidomics Mass Spectrom mass spectrometry matrix measured metabolic profiling metabolite identification metabolomics data metabolomics studies methanol methods microbial microfluidic molecular molecules multivariate nontargeted obtained pathways performed plant Proteome Proteome Res quantitative quenching retention sample preparation sensitivity separation signal Smilde solvent species spectra spectrum stationary phase targeted techniques tissue TOFMS typically uncertainty untargeted urine validation variables variation Verpoorte