Metastasis Research Protocols
Susan A. Brooks, Udo Schumacher
Humana Press, Jul 20, 2001 - Medical - 336 pages
Metastasis Research Protocols describes in detail all the methods needed to investigate why and how metastasis occurs. Volume I presents a comprehensive collection of established and leading-edge techniques for analyzing the expression of key molecules and for examining their production at the genetic level. Volume I focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression. The traditional methods range from the histopathological and the immunocytochemical to SDS-PAGE, Western blotting, and enzyme zymography. Newer and more specialized techniques for analyzing the genetic aspects of metastasis include in situ hybridization to localize mRNAs, FISH, CGH, methylation analysis of CpG islands, RT-PCR, differential display. Volume II presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade. These systems include cell migration assays, assays for matrix degradation enzymes, basement membrane degradation assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models for metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis.
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Flow Cytometric Measurement of Cell Proliferation
Contents of Companion Volume
Immunomagnetic Cell Separation
19 other sections not shown
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aggregation angiogenesis angiogenic animal Anticancer antigen approx beads blood vessels breast cancer buffer cancer cells Cancer Res carcinoma cells cell culture cell lines cell suspension centrifuge chamber chick clinical clone collagen colon cancer colorectal containing culture medium Cytometry developed distilled water eggs embryo endothelial cells ethanol experimental expression filter flask flow cytometry fluorescence forceps gene glass growth factor Hoffman human tumor implantation Incubate inhibitor injection integrin liver lung lymph nodes mammary Mareel Matrigel matrix melanoma Metast metastasis metastasis models Metastasis Research Protocols metastatic Methods micrometastases microscope microslide molecules Natl needle Note nude mice nude mouse number of cells orthotopic phenotype pipet plate primary antibody primary tumor prostate cancer protein remove resuspend SCID mouse sections serum slides solution staining sterile store at 4°C studies subcutaneous Subheading syringe transfection transgenic transplantation trypsin trypsin-EDTA tube tumor cells vascular vitro Wash xenograft