Methodologies for Metabolomics: Experimental Strategies and Techniques

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Norbert W. Lutz, Jonathan V. Sweedler, Ron A. Wevers
Cambridge University Press, Jan 21, 2013 - Medical - 627 pages
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Metabolomics, the global characterization of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways, and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites including nuclear magnetic resonance and mass spectrometry are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples, and diseases, ranging from in vivo metabolomics in humans and animals, to in vitro analysis of tissue samples, cultured cells and biofluids.
 

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Contents

Exploring the Human Metabolome by Nuclear Magnetic
3
Methodological Requirements for Lipidomics Research
30
Santiago DiazMoralli Markus Godejohann
48
Biological Methods for Metabolic Research
54
Mass SpectrometryBased Methodologies for SingleCell
119
Laser Ablation
140
Approaches for Natural Product Detection and Structural
174
From Sampling
205
Nuclear Magnetic Resonance Analysis and Genetic Metabolic
299
Lipid Profiling in Health and Disease
317
METABOLOMIC NUCLEAR MAGNETIC
333
Phospholipidomics by Phosphorus Nuclear Magnetic Resonance
377
Carbon13 Nuclear Magnetic Resonance for Analysis
415
Metabolomic Magnetic Resonance Spectroscopy of Human
472
Reproducible Sample Preparation and Spectrum Acquisition
496
Assignment Strategies for Nuclear Magnetic Resonances
525

NUCLEAR
225
Chemometric Methods in Nuclear Magnetic ResonanceBased
244
Nuclear Magnetic ResonanceBased Saliva Metabolomics
271

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About the author (2013)

Norbert W. Lutz is Research Professor at the School of Medicine at the University of Aix-Marseille, France. He has previously held positions at the Johns Hopkins University School of Medicine, the German Cancer Research Center, the University of Arizona Cancer Center and other internationally renowned research institutions. His research is primarily focused on the analysis of metabolic processes in cancer and in neurological diseases. His papers have appeared in Analytical Chemistry, Annals of Neurology, PLoS ONE, International Journal of Cancer, AIDS, Metabolomics, NMR in Biomedicine and other journals.

Jonathan V. Sweedler is the James R. Eiszner Family Professor of Chemistry and the Director of the School of Chemical Sciences at the University of Illinois at Urbana-Champaign, USA. His major research efforts involve developing new approaches for small-volume peptidomics and metabolomics and the application of these technologies to study novel neuro-chemical pathways. Professor Sweedler has published more than 350 manuscripts, book chapters and reviews and has presented 350 invited lectures relate to research in these areas. He is Editor-in-Chief of Analytical Chemistry.

Ron Wevers is head of the laboratory of Genetic Endocrine and Metabolic Diseases of the Radboud University Nijmegen Medical Centre in the Netherlands. He has a chair in Clinical Chemistry of Inborn Errors of Metabolism. Professor Wevers is also a member of the Dutch Health Council. He has co-authored more than 300 peer-reviewed papers and many book chapters and wrote a handbook on body fluid NMR spectroscopy in patients with inborn errors of metabolism.

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