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Method Choice and Development
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3-carotene A0AC absorbance activity adjust aliquot amount Anal analysis analytical animal ascorbic acid Assoc autoclave automated basal Biochem Biol biological biotin blank buffer calculated carotene carotenoids casei cell centrifuge Chem chemical chick choline Clin cobalamins color column compounds concentration conjugase containing dehydroascorbic acid detector determination of vitamin diet Dissolve eluted enzyme ethanol ether extract factor filter filtrate fluorescence fluorometric folacin folate folic acid foods hexane HPLC incubation injection isomers jug/mL liquid chromatography liver materials measured medium menadione method microbiological assay milk mixture mL with H20 mobile phase Na0H niacin Nutr Nutrition oxidation pantothenic acid peak height Pipette plasma Prepare procedure protein provitamin quantitation radioassay rats Reagent retinol riboflavin sample saponification separation serum sodium acetate solution Reagent solvent spectrophotometer standard curve standard solution stock solution temperature thiamin tissues titration tocopherols tubes vitamin B,2 volumetric flask weight