Methods On Glycoconjugates
By guiding the reader toward answering basic questions on the structural analysis of glycoconjugates using conventional techniques, this book studies methods such as chemical cleavage, molar composition, use of enzymes, use of lectins and typical fractionation procedures such as HPLC, GLC, TLC, and other chromatographic systems. More sophisticated techniques such as NMR or MS are described to give the reader the essential information needed to adapt procedures to his/her requirements.
This book will serve as a practical manual designed for scientists not familiar with glycoconjugate methodologies, but whose research work requires contact with analytical problems or metabolic investigations in glycobiology. It is the result of twenty years of experience of summer schools held in a laboratory environment and will acquaint the reader with widely used techniques in the area rather than cataloguing an exhaustive list of methods. This will help any biochemist to develop an experiment with ease.
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Colorimetric micro methods
Isolation of glycopeptides and glycosylation sites
Gelfiltration and thinlayer chromatography of oligosaccharides
Supercritical fluid chromatography SFC of oligosaccharides
Methylation analysis of carbohydrates
Structure Determination by Physicochemical and Biochemical Methods
Use of exoglycosidases
Metabolic labelling of animal cell culture
Glycosyltransferases involved in the processing of Nglycosidically
acceptor acetic acid activity added affinity chromatography agglutinin Anal analysis assay biantennary Biochem Biol buffer pH carbohydrate cell charides Chem CMP-NeuAc concentration containing Debray detection determination dissolved electrophoresis eluted endo enzyme enzyme source extract fractionation fucose galactose GlcNAc glucose glycans glyco glycoconjugates glycopeptides glycoproteins glycosidic glycosylation glycosyltransferase gosaccharides gradient HPLC hydrazinolysis hydrolysis immobilized lectins incubation interaction ions isolated Kobata labelled lectin lectin column linkage liquid chromatography Man(o mannose mass spectrometry membrane methanol method methyl mixture ml/min moiety molecular monosaccharides Montreuil N-acetyl N-acetylglucosamine N-acetyllactosaminic type N-glycosylpeptides NaOH neoglycolipids NeuAc neuraminic acid neutral non-reducing obtained oligomannosidic type oligosac oligosaccha oligosaccharides peptides procedure protein purification radioactive reaction is stopped reagent residues room temperature saccharides sample separation sequences sialic acid sialylated sialyltransferase sodium solution solvent specificity structure substrate sugar sugar-nucleotides terminal tion tubes UDP-GlcNAc washed