Methods On Glycoconjugates

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CRC Press, Jul 1, 1995 - Medical - 215 pages
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By guiding the reader toward answering basic questions on the structural analysis of glycoconjugates using conventional techniques, this book studies methods such as chemical cleavage, molar composition, use of enzymes, use of lectins and typical fractionation procedures such as HPLC, GLC, TLC, and other chromatographic systems. More sophisticated techniques such as NMR or MS are described to give the reader the essential information needed to adapt procedures to his/her requirements.
This book will serve as a practical manual designed for scientists not familiar with glycoconjugate methodologies, but whose research work requires contact with analytical problems or metabolic investigations in glycobiology. It is the result of twenty years of experience of summer schools held in a laboratory environment and will acquaint the reader with widely used techniques in the area rather than cataloguing an exhaustive list of methods. This will help any biochemist to develop an experiment with ease.
 

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Contents

Colorimetric micro methods
27
Isolation of glycopeptides and glycosylation sites
41
Gelfiltration and thinlayer chromatography of oligosaccharides
63
Supercritical fluid chromatography SFC of oligosaccharides
79
Methylation analysis of carbohydrates
93
Structure Determination by Physicochemical and Biochemical Methods
135
Use of exoglycosidases
159
Metabolic labelling of animal cell culture
179
Glycosyltransferases involved in the processing of Nglycosidically
201
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