MicroRNAs as Tools in Biopharmaceutical Production

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Niall Barron
Springer Science & Business Media, Nov 15, 2012 - Medical - 126 pages
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Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

 

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Contents

Chapter 1 A General Introduction to MicroRNAs Their Investigation and Exploitation in CHO Cell Lines
1
Chapter 2 Biogenesis of Mammalian miRNA
14
Chapter 3 Target Prediction Algorithms and Bioinformatics Resources for miRNA Studies
29
Chapter 4 The CHO miRNA Transcriptome
49
Chapter 5 MicroRNAs as Engineering Targets
65
Insights from Neuroblastoma Studies
86
Chapter 7 MicroRNA Detection Methods for Mammalian Cell Lines and Their Applications in Therapeutic Protein Production
99
Chapter 8 Manipulating Levels of Specific MicroRNAs in Mammalian Cells
112
Index
122
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