Microarrays for the Neurosciences: An Essential Guide

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Daniel H. Geschwind, Jeffrey P. Gregg
MIT Press, 2002 - Medical - 332 pages
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The effort to sequence the human genome has generated a new discipline, "functional genomics," or the study of the relationship between the genetic code and its biologic potential. Gene expression studies are made possible not only by the decoding of the human genome, but by the development of new technologies. The preeminent technology in this area, DNA microarrays, is helping to revolutionize the field of neuroscience. Rather than looking at one gene at a time, researchers using DNA microarrays can monitor the expression patterns of large numbers of genes simultaneously. Bridging the traditional gap between molecular neurobiology and systems neurobiology, DNA microarray technology has the potential to elevate molecular genetic studies of the nervous system to the system level.

This book provides a comprehensive guide to the use of DNA microarrays in neuroscience and provides approaches that are applicable to other complex biological systems. Human nervous system tissue is remarkably complex. The number of cell types, the architecture, the developmental program, and the importance of environmental factors in development and functioning all pose particular challenges to the researcher using gene expression studies. After an overview of the technology, the book discusses array scanning and image application, statistical methods for array analysis, specific applications of gene expression studies in the central nervous system, the use of postmortem human tissue, and novel methods for using microarray data to develop hypotheses about regulatory networks.
 

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Contents

An Overview
1
Microarray Scanning and Data Acquisition
29
Microarray Image and Data Analysis
63
Statistical Inference in Array Genomics
109
CustomBuilt cDNA Arrays for the Study of Neuronal Differentiation
157
Development of Microarrays to Study Gene Expression in Tissue
237
Custom cDNA Microarrays Derived from Subtracted Libraries
255
Tracing Genetic Information Flow from Gene Expression to Pathways
273
Appendix A Informatics and Web Resources
301
Abbreviations
315
Copyright

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About the author (2002)

Daniel H. Geschwind is Assistant Professor of Neurology and Director of the Neurogenetics Program at the University of California at Los Angeles School of Medicine.

Jeffrey P. Gregg is Assistant Professor of Pathology and Director of Molecular Pathology at the University of California at Davis School of Medicine.

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