Modern Biophysical Chemistry: Detection and Analysis of Biomolecules

Front Cover
John Wiley & Sons, Sep 10, 2015 - Science - 360 pages
0 Reviews
This updated and up-to-date version of the first edition continues with the really interesting stuff to spice up a standard biophysics and biophysical chemistry course. All relevant methods used in current cutting edge research including such recent developments as super-resolution microscopy and next-generation DNA sequencing techniques, as well as industrial applications, are explained. The text has been developed from a graduate course taught by the author for several years, and by presenting a mix of basic theory and real-life examples, he closes the gap between theory and experiment.
The first part, on basic biophysical chemistry, surveys fundamental and spectroscopic techniques as well as biomolecular properties that represent the modern standard and are also the basis for the more sophisticated technologies discussed later in the book. The second part covers the latest bioanalytical techniques such as the mentioned super-resolution and next generation sequencing methods, confocal fluorescence microscopy, light sheet microscopy, two-photon microscopy and ultrafast spectroscopy, single molecule optical, electrical and force measurements, fluorescence correlation spectroscopy, optical tweezers, quantum dots and DNA origami techniques. Both the text and illustrations have been prepared in a clear and accessible style, with extended and updated exercises (and their solutions) accompanying each chapter.
Readers with a basic understanding of biochemistry and/or biophysics will quickly gain an overview of cutting edge technology for the biophysical analysis of proteins, nucleic acids and other biomolecules and their interactions. Equally, any student contemplating a career in the chemical, pharmaceutical or bio-industry will greatly benefit from the technological knowledge presented. Questions of differing complexity testing the reader's understanding can be found at the end of each chapter with clearly described solutions available on the Wiley-VCH textbook homepage under: www.wiley-vch.de/textbooks
 

What people are saying - Write a review

We haven't found any reviews in the usual places.

Selected pages

Contents

Cover
Introduction
Basic Methods in Biophysical Chemistry
Figure 7 2
Figure 5 13
Environments Vibrational Excitation and
Figure 4 8
Figure 9 6
Figure 2 1
Figure 3 1
Figure 4 1
Chiroptical and Scattering Methods
Table 2 1
Magnetic Resonance Techniques
Table 4 1
Mass Spectrometry

Figure 10 1
FranckCondon Principle 1 5WhatHappens After Molecules have Absorbed
Figure 3 6
Figure 6 1
Figure 7 1
Basic Fluorescence Techniques
Figure 5 7
Figure 1 16
AdvancedMethods inBiophysical Chemistry
SuperResolution Fluorescence Microscopy
Table 10 1
Figure 1 1
Special Techniques
HighThroughput Screening Bibliography
Index

Other editions - View all

Common terms and phrases

About the author (2015)

Peter J. Walla is a group leader at the Max-Planck-Institute for Biophysical Chemistry in Gottingen (Germany), and Professor for Biophysical Chemistry at the University of Braunschweig. Having obtained his academic degrees from the Universities of Heidelberg and Gottingen, Professor Walla did postdoctoral research at the University of California at Berkeley, and headed a department in a Biotech company (now part of Bayer HealthCare) co-founded by Nobel Laureate Manfred Eigen, before taking up his current position. He has very broad research expertise in biophysical chemistry and is author of high-ranking publications covering techniques such as super-resolution microscopy, single molecule spectroscopy and two-photon ultrafast spectroscopy and their application in fields like neuroscience or photosynthetic light harvesting and energy conversion.

Bibliographic information