Molecular Diagnostics: For the Clinical Laboratorian

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William B. Coleman, Gregory J. Tsongalis
Springer Science & Business Media, 2006 - Medical - 567 pages
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In 1994 I coedited a book, Molecular Pathology: Ap- efforts. It should be evident that molecular pathology and proaches to Diagnosing Human Disease in the Clinical diagnostics impact almost every conceivable subspecialty Laboratory, that coined the phrase “molecular pathology” in laboratory medicine and, particularly in the case of to refer to applications of molecular biology in the tradi- pharmacogenomics, have led to the development of new tional areas of laboratory medicine. That compilation of areas of investigation. clinical molecular techniques included 11 chapters and an What is less evident is the burden placed upon the epilogue on “New Directions for the Clinical Laboratory. ” laboratorians directing these efforts. Issues regarding Chapter headings included: automation, neoplasia, heri- training, certification, continuing education, and re- table diseases, and infectious diseases. bursement (just to name a few) have demanded enormous Three years later, in 1997, Bill Coleman and Greg Tson- time and effort from professional organizations and g- galis edited Molecular Diagnostics: For the Clinical ernmental agencies. In addition, because conventional Laboratorian, which contained 18 chapters and introduced approaches cannot always be applied to molecular di- the new areas of RT-PCR and In Situ PCR, and included nostics, unique solutions for quality assurance and quality a section on gene therapy. Now, ten years after the intro- control must be developed. These challenges have eng- duction of molecular pathology, we are looking at the dered committees, subcommittees, taskforces, and second edition of Bill Coleman and Greg Tsongalis' text.
 

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Contents

An Historical Perspective on the Clinical Diagnostic Laboratory
3
An Overview of Nucleic Acid Chemistry Structure and Function The Foundations of Molecular Biology
13
Extraction of Nucleic Acids
25
Nucleic Acid Blotting Techniques Theory and Practice
31
The Polymerase Chain Reaction
47
Bioinformatics ComputerBased Approaches to Genetic Analysis
57
PCRBased Methods for Mutation Detection
65
Alternative Methods for Amplified Nucleic Acid Testing
75
Molecular Mechanisms of Endocrine Disorders
281
Molecular Pathogenesis of Cardiovascular Disease
295
Molecular Diagnostics in Coagulation
311
Cystic Fibrosis
321
Prenatal Genotyping for Identification of Fetuses at Risk for Immune Cytopenic Disorders
329
Personalized Medicine
341
Molecular Pathogenesis of Human Cancer
349
Application of Molecular Diagnostics to Hereditary Nonpolyposis Colorectal Cancer
375

Electrophoretic Methods for Mutation Detection and DNA Sequencing
85
SingleNucleotide Polymorphisms Testing DNA Variation for Disease Association
111
Microarray Approaches to Gene Expression Analysis
121
Methods for Analysis of DNA Methylation
149
Flow Cytometry
163
Medical Cytogenetics
173
Fluorescence In Situ Hybridization A Major Milestone in Luminous Cytogenetics
189
Immunohistochemistry
203
Laser Capture Microdissection
219
Framework for Quality Assurance in Molecular Diagnostics
227
Verification of Molecular Assays
237
Standards and Standardization of Molecular Diagnostics
243
LaboratoryDeveloped Tests in Molecular Diagnostics
247
An Overview of Molecular Genetics
259
Genetic Basis of Neurologic and Neuromuscular Diseases
267
Molecular Genetic Applications to the Diagnosis of Lymphoma
393
Molecular Genetic Abnormalities in Acute and Chronic Leukemias
415
Molecular Testing for Chlamydia trachomatis and Neisseria gonorrhoeae
437
Human Papillomavirus
447
Molecular Diagnostics for HIV1
453
Hepatitis C
461
Cytomegalovirus
473
HLATyping Using Molecular Methods
485
Molecular Analysis for Forensic Casework and Parentage Testing
495
Molecular Assessment of Bone Marrow Transplant Engraftment
511
Use of DNABased Identity Testing for Specimen Identification
517
Genetic Counseling Considerations in Molecular Diagnosis
525
Ethical Social and Legal Issues Related to Molecular Genetic Testing
545
Index
555
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My name is William B. Coleman. Currently I am 32 years old originally from Charlotte, NC. I'm the Author of the novel, "Bless Da Child Dat Holds His Own" and "No the Hell She Didn't." In order to view my novels cover page and synopsis, Google my name and book title. I simply love to write. I feel as though I was blessed with the talent to use my imagination, create characters and share with the world my stories. I'm very versatile and professionally good at what I do. Along with writing novels, I definitely read a lot, personal fitness trainer, write screen plays, stage plays and I've just completed a Children's Entrepreneur book. I'm all for accomplishing goals and striving for success. Slowly but surely, my hard work is now finally starting to pay off. I can't stop and I definitely won't stop pushing myself to succeed. I have a mind that never stops working. The brain I have is loaded with many ideas and plans. I keep myself occupied and I'm never bored because I'm always working on something which will help me in the future. Nothing comes to those who wait for handouts. I believe no human who has mobility, in good shape and good health should wait for anything. Get up off your behind and get it! Stop depending on the finances of others and make things happen for yourself. If they succeeded, so can you. I sincerely believe in getting things done. If you have any questions or any comments about any of my novels, feel free to speak your mind or you can contact me via email at wbcoleman81@gmail.com or PenPushingPublishingLLC@gmail.com Thanks. Other Novels by William B. Coleman available on Amazon. Paperback or eBook Custom Made 1 Custom Made 2 No The Hell She Didn't Bless Da Child Da Holds His Own & Suzie's Famously Funny Cupcakes & Ben's Bodacious Fruit Bowls ( Entrepreneur Book For Children ) Coming Soon Am I My Sister's Keeper Custom Made 3 Not A Born Mistake ( The William B. Coleman Story ) Bless Da Child Dat Holds His Own 2 No The Hell She Didn't 2 Ms. Demeanor

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