Methods in Pharmacology, Volume 7; Volume 1993

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Springer Science & Business Media, Sep 30, 1993 - Medical - 277 pages
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In this volume of Methods in Pharmacology we have brought together experts in the field of ion channels to provide the reader with an in-depth view of current biochemical and electrophysiological methods. The focus is on cation-selective ion channels (voltage controlled Of ligand controlled) that are of special pharmacological interest. The con­ tents are divided into three sections that exemplify {I) molecular and biochemical characterization of ion channels, (II) functional analysis of ion channels (after recon­ stitution or expression, or in cells), and (III) specific methods and tools. The editors thank the contributors for their cooperation in assembling this volume. We are convinced that the reader will enjoy the chapters, written by leading scientists in their respective disciplines, as much as we did. Hartmut Glossmann Jarg Striessnig Innsbruck, Austria vii Contents I MOLECULAR AND BIOCHEMICAL CHARACTERIZA nON OF ION CHANNELS 1 Purification and Pharmacological Analysis of the Omega-Conotoxin 3 GVIA Receptor from Rat Brain Maureen W McEnery 2 Molecular Characterization of the Charybdotoxin-Sensitive, 41 High-Conductance, Calcium-Activated Potassium Channel Margarita Garda-Calvo, Gregory J. Kaczorowski, and Maria L. Garda 3 Characterization of the Sulfonylurea-Sensitive ATP-Modulated 61 Potassium Channel Henri Bernardi and Michel Lazdunski II FUNCTIONAL ANALYSIS OF ION CHANNELS 4 Xenopus Oocytes as an Expression System for Ion Channels 79 Alan L. Goldin ix x Contents 5 Reconstitution of Muscle Calcium Channel Function in Bilayer 99 Membranes: From the First Steps to Results Dieter J. Pelzer, Terence F.
 

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Contents

Purification and Pharmacological Analysis of the OmegaConotoxin GVIA Receptor from Rat Brain
3
Molecular Characterization of the CharybdotoxinSensitive HighConductance CalciumActivated Potassium Channel
41
Characterization of the SulfonylureaSensitive ATPModulated Potassium Channel
61
Functional Analysis of Ion Channels
77
Xenopus Oocytes as an Expression System for Ion Channels
79
Reconstitution of Muscle Calcium Channel Function in Bilayer Membranes From the First Steps to Results
99
Approaches to Studying the Interaction between GProteins and VoltageDependent Ca² Channels
141
Influence of GABA on Potassium Channels in Hippocampal Neurons
165
Estimation of Drug Affinities for Calcium Channel Conformational States
189
Specific Methods and Tools
221
Production of Monoclonal Antibodies against LowAbundance IonChannelAssociated Proteins in Neuronal Tissue
223
A Highly Porous Sodium Dodecyl Sulfate Polyacrylamide Gel System A Versatile Technique for Protein Structure and Function Study
237
Synthesis and Properties of Diazipine a Novel Photoaffinity Probe for Calcium ChannelLinked Dihydropyridine Receptors
255
Index
267
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Page 253 - Western Blotting": Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated Protein A. Anal. Biochem.
Page 253 - UK (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J. Biol. Chem.

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