Molecular Genetic Analysis of Populations: A Practical Approach
A. Rus Hoelzel
IRL Press at Oxford University Press, 1998 - Science - 445 pages
Methods enabling the direct study of genetic variation in natural populations have improved considerably. The new edition explores these updated techniques in DNA analysis and provides a revised and refined laboratory guide to investigating variation in DNA molecules.
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Total DNA isolation
Mitochondrial DNA isolation separation
28 other sections not shown
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acrylamide agar agarose gel alleles allozyme amplification analysis annealing Applied Biosystems bands caesium chloride cells centrifuge Chapter cloning concentration containing cosmid denatured described in Protocol detection digestion dilution DNA fingerprinting DNA fragments DNA samples DNA sequence EDTA Equipment and reagents ethanol ethidium bromide filter fraction gene genetic genomic DNA genotype gradient hybridization incubate insert isolation lambda ligation lncubate loading loci locus membrane method mg/ml microcentrifuge tube microsatellite minisatellite molecular mtDNA multilocus mutation NaCl NaOH Nucleic Acids Nucleic Acids Res nucleotide oligonucleotide PCR primers PCR product pellet pipette plasmid plates polymorphism population precipitate probe protein remove restriction enzyme Resuspend room temperature sodium acetate solution source DNA Southern blots species SSCP staining step sterile supernatant Taq DNA polymerase techniques template tion tissue Tris-HCl pH variation vector vector DNA volume wash
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