Molecular techniques in taxonomy
Godfrey M. Hewitt, A. Johnston, J. Peter W. Young, North Atlantic Treaty Organization. Scientific Affairs Division
Springer-Verlag, 1991 - Science - 410 pages
Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing.
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Exploiting Conserved and Variable Regions
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Acad Sci USA Acids Res alleles allozyme amplification analysis bands biology buffer buzzatii Caccone Cell Centrifuge characters chloroplast chromosome cladistic codon colonization concentration conserved cpDNA CsCl differentiation digestion divergence DNA fingerprinting DNA sequences DNA-DNA hybridization Dolichopoda Doyle Drosophila melanogaster EDTA enzyme ethanol eukaryotic evolutionary extraction fraction gene genetic gradient homogenate Incubate individuals isolation labelled loci males method minisatellite mitochondrial DNA mitochondrial genome moiavensis Mol Biol Evol Mol Evol molecules morphological mtDNA mutation Natl Acad Sci nuclear DNA nucleotide nucleotide sequence pattern pellet phylogenetic phylogeny plant polymerase polymorphism populations Powell primer probes Proc Natl Acad protein protocol reaction region relationships repeat Resuspend ribosomal RNA rRNA sample satellite DNA Sbordoni selection solution speciation sperm length Spin sterile stranded structure substitutions sucrose supernatant taxa TEACL Techniques in Taxonomy temperature tree tRNA tube Urchin variability variation volvulus