Cancer and the mechanisms of its development are receiving increasing research interest. New genes are being isolated almost daily, Fundamental to those studies is the ability to detect mutations. Mutation detection is time consuming and expensive and thus should be undertaken with a thorough knowledge of the alternative methods. This book aims to critically review the field, refer to methodology and protocols, and give illustrative examples as well as put the whole emerging field in context, including ethical considerations.
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Academy of Sciences allele APC gene assay base changes base pair cancer cDNA cells CFTR chemical cleavage cleavage method cleaved Clinical codons complementary conformation polymorphism Cotton cystic fibrosis deletions denaturing gradient gel detect mutations detection of mutations detection of point detection rate developed DGGE diagnostic laboratory digestion DNA sequencing dot blot duplex dystrophin exons fluorescence fragments GC clamp genomic DNA gradient gel electrophoresis homoduplexes Human Genetics Human Molecular Genetics Human Mutation hydrolink known mutations labelled large number ligase ligase chain reaction melting mRNA multiplex muta mutant and wild mutant molecules mutation detection mutation scanning National Academy Nucleic Acids Research oligonucleotide patients PCR Methods PCR product point mutations polymerase chain reaction polymorphism Proceedings protein recently reproduced with permission restriction enzyme RNAse sample scanning methods screening single strand SSCA SSCP strategy Table target temperature tion tube tumour variation wild type