Mutation Detection: A Practical Approach
Richard G. H. Cotton, Edward Edkins, Sue Forrest
IRL Press at Oxford University Press, 1998 - Medical - 242 pages
Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field.
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Singlestrand conformation polymorphism
Singlestranded conformation polymorphism
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Acad acrylamide agarose gel allele allele-specific analysis annealing assay bands base pair beads biotinylated buffer capillary cDNA Centrifuge clamp cleavage concentration cycles denaturant DGGE diluted DNA polymerase DNA sequencing dNTPs EDTA Endo VII enzyme Equipment and reagents ethanol ethidium bromide exon factor IX Figure filter formamide fragments gel electrophoresis gene Genet genomic genomic DNA gradient haplotype heteroduplex homoduplex hybridization hydroxylamine immobilized probe Incubate lane ligation manifold support melting meltmap method MgCl2 microtitre plate mismatch mobility molecular molecules mRNA multiplex mutation detection Natl normal Nucleic Acids Res nucleotide oligonucleotide osmium tetroxide PASA PCR amplification PCR products pellet plasmid polymorphisms Proc protein Protocol psoralen resuspend RNA probe RNase room temperature segment sensitivity separation single base single-stranded solid phase solution specific splice SSCP strand supernatant target template tion transcription Tris-HCl pH tube U/ul wash wild-type yeast