Nonisotopic DNA Probe Techniques
Larry J. Kricka
Academic Press, Dec 2, 2012 - Science - 358 pages
Recently many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as 32phosphorous and 125iodine. This book brings all of these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited to their application and to use it to maximum advantage by following the straightforward instructions provided.
This book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluorescent, and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapterpresents useful background information and detailed, step-by-step, easy-to-follow, experimental procedures for labeling and detection.
Gives extensive practical information
Covers major types of nonisotopic labels and procedures
Presents background information for each method
Provides strategies and detailed experimental procedures for labeling and detecting DNA sequences by
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AE-probe alkaline phosphatase ampliﬁcation AMPPD Anal antibody applications assay format Biochem bioluminescent biotin biotin-labeled biotinylated biotinylated probes Bronstein cells chelate Chem chemical chemiluminescence Clin colorimetric complex conjugate containing denatured detection of nucleic detection systems Diamandis differential hydrolysis digoxigenin dilution DNA hybridization DNA probes double-stranded EDTA energy transfer enzymatic enzyme ester Eu-labeled europium ﬁlm ﬁlter ﬁnal concentration ﬁrst gene hapten hybridization assays hybridization buffer hybridization probes hydrolysis immunoassays Incubate Kricka labeled probes luminescent luminol luminometer membrane method modiﬁed molecular molecules NaCl nitrocellulose nonisotopic nonradioactive nucleic acid hybridization nucleic acid probes Nucleic Acids Res nucleotide oligomer oligonucleotide probes phosphate polymerase polynucleotide primers probe concentration procedure protein protocol puriﬁcation quenching reaction reagent room temperature sample sandwich hybridization sensitivity signal single-stranded slides sodium solution Southern blots speciﬁc strands streptavidin substrate synthesis target DNA technique tion tube W0 Patent wash