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Acta active transport acylphosphatase Ahmed and Judah ATP hydrolysis ATP-dependent binding ATPase activity ATPase preparation ATPase reaction binding energy binding of sodium Biochem Biol Biophys Ca-ATP calcium calcium and ouabain cardiac glycosides cation cation transport cell membrane centrifugation Charnock Chem decreased effect of ouabain effect of potassium enzymatic enzyme ethacrynic acid follow first-order kinetics Garrahan and Glynn Hill equation Hoffman hydrolysis of ATP I.M. Glynn increased incubation inhibited by calcium inhibited by ouabain inhibitor inhibitory effect interaction ions kidney ATPase kidney cortex magnesium medium Mg-ATP microsomal Nagano Nakao observed oligomycin Opit ouabain phosphate phosphorylated phosphorylated intermediate Physiol postulated potassium concentration presence of sodium Proc protein R.L. Post red blood cells rubidium Schatzmann Sjodin and Beauge Skou slices slopes sodium and potassium sodium binding sodium concentration sodium efflux sodium pump sodium-potassium ATPase stimulation studied substrate tissue total binding capacity transport of sodium transport system Wallach