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actively motile spirochetes aerobioally agar alkaline alkaline pyrogallol anaerobic anaerobio ascites fluid autoclaved bacteria beef blood bouillon c.o. serum chetes coagulated horse serum cultivation days after inoculation dium employed extract fair growth Fibrin gelatinized horse serum gram-positive growth of spirochetes hormone broth human condylomas hydrogen impure cultures incubated at 35 infection intratesticular kidney tissue Kolmer liver medium method minutes mixed culture monkey neutralized cysteine hydrochloride Noguchi non-motile spirochete normal rabbit normosal Novy jar o.c. distilled water o.c. serum o.o. broth oil was added old pure culture one-half hour oould orchitis papules peptone pipette previously heated primary human lesions purified pyrogallol Rabbit inoculations rabbit kidney rabbit serum refringens saline saprophytic spirochetes Schereschewsky serum heated serum water Sheep serum Shmamine sodium chloride spiro Spirocheta pallida spleen sterile paraffin oil strain subcultures syphilitic rabbit testicle syphilitio material syphiloma test tubes testicle emulsion Treponema pallidum tubes were incubated tubes were set vitro workers