PCR: essential techniques
Essential Techniques Series PCR is one of the most important techniques in modern molecular biology and many variants, based on DNA amplification, have been developed. This book provides detailed practical guidance on both the established and new applications of PCR for a wide range of disciplines, for example gene analysis, DNA sequencing, forensics and diagnostics. It will be essential reading for all those using PCR, whatever their field of interest. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.
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00T polymerase 0aTl 0epartment 0icropipettor plus tips 0ineral 0niversity 0uman 3ciences agarose gel aliquot alleles analysis annealing autoclaved buffer cDNA cells centrifuge cloning concentration containing cycles daT0 denaturation detection digestion diluted distilled water DNA sequencing dNTPs electrophoresis enzyme Eppendorf tube Equipment ethanol ethidium bromide exonuclease extract fragment gene Genetics genomic hybridization Incubate jjlI kinase labeled ligase ligation m0 Tri/Trl m0 TTT master mix membranes mg/ml microcentrifuge tube microtiter mineral oil mixture mutation Notes This procedure Nucleic Acids Res oligo oligonucleotide optimized PCR amplification PCR primers PCR product PCR reaction pellet pipette plasmid precipitation probe procedure will take Protocol purification reaction mix Reagents remove Resuspend reverse transcriptase ribooligonucleotide room temperature RT-PCR sample single-stranded solution spin sterile synthesis take approximately Taq DNA polymerase Taq polymerase target technique template termination thermal cycler transcription trehalose tubes 0.T ml Wash