PCR Technology: Principles and Applications for DNA Amplification
Polymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for.
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PART ONE BASIC METHODOLOGY
The Design and Optimization of the PCR
PCR Amplification of Specific Sequences from
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Acad Acids activity added addition alleles allow amount amplification analysis annealing applications approach assay automated base blood buffer cDNA cells changes Chapter cloned combined complex concentration containing cycles deletion denaturant described detection determined DGGE direct discussed disease DNA fragments DNA sequences dNTP domain effect efficient electrophoresis enzyme Erlich example extension Figure GC-clamp gene genetic genomic gradient heating human hybridization identified increase incubation individual isolation known labeled lane limited locus melting method molecular Mullis mutations Nature nucleotide observed obtained oligonucleotide PCR product performed pmol polymorphism position possible preparation present primers probes problem Proc procedure Protocol reaction recombination region restriction Saiki samples Science separate shown single specific sperm step strands studies synthesis Taq DNA polymerase Taq polymerase temperature template tube usually yield