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PART ONE BASIC METHODOLOGY
TD The Design and Optimization of the PCR
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Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing Arnheim assay automated B-globin base cDNA cell Cetus chromosome clones concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Eh Eh Eh Eh H Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers method MgCl2 mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers oligonucleotide probes PCR amplification PCR analysis PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf single sperm Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumor