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Milestones for the Chemical Synthesis of Proteins
Inhibition of AngiotensinConverting Enzyme Activity by Syn
Identification of AngiotensinConverting Enzyme Inhibitory
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AcOH amide amino acid amino acid analysis amino acid residues amino acid sequence amphiphilic analogs assay binding Biochem Biol biological activities Biophys cells Chem cleaved coacervation column compounds concentration conformation coupling CuCl2 dimers disulfide Edman degradation effect elution endothelin enkephalin enzyme ester final deprotection flow rate fragments Fujii gramicidin HBVp HCl/CH3CN HOBt HPLC hydrolysis hydrophobic inhibition inhibitory insulin interaction Japan Kangawa Kimura lanthiopeptin lipid liposomes lysinoalanine M.Ueki melittin membrane method methyl ml/min molecular N-terminal natriuretic peptides obtained Osaka oxidation Paim pardaxin pBNP peak Peptide Chemistry 1988 peptide synthesis phosphorylated porcine BNP potent prepared presence primary structure procedure protected peptide protecting groups Protein Research Foundation purified racemization reaction reagent receptor resin Science Sephadex showed shown in Fig solid phase synthesis solution solvent spectra substrates synthetic peptides Table vasorelaxant Yajima Yanaihara yield