Phage Display: A Laboratory Manual, Page 532

Front Cover
CSHL Press, 2004 - Science - 736 pages
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Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
 

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Contents

1 Filamentous Phage Biology
1-1
2 Phagedisplay Vectors
2-1
3 Antibody Libraries
3-1
4 Peptide Libraries
4-1
5 Functional Domains andScaffolds
5-1
6 Gene Fragment Libraries and Genomic and cDNA Expression Cloning
6-1
Amplification of Antibody Genes
6-12
Immunization RNA Preparation and cDNA Synthesis
8-1
17 Screening Peptide Libraries
17-1
18 Analysis of Phageborne Peptides
18-1
19 Construction and Use of pIIIdisplayed Peptide Libraries
19-1
20 Construction and Selection from Gene Fragment Phagedisplay Expression Libraries
20-1
21 Construction and Selection from cDNA Phagedisplay Expression Libraries
21-1
22 In Vivo Selection of Phagedisplay Libraries
22-1
23 Cellsurface Selection and Analysis of Monoclonal Antibodies from Phage Libraries
23-1
App 1 Useful Information
AJ-1

PCR Amplification and Assembly of Light and and Heavychain Coding
9-1
10 Selection from Antibody Libraries
10-1
11 Analysis of Selected Antibodies
11-1
12 Production and Purification of Fab and scFv
12-1
13 Antibody Engineering
13-1
Peptide Libraries
13-16
15 General Phage Methods
15-1
16 Production of Peptide Libraries
16-1
App 2 Recipes
AJ-11
App 3 General Procedures
AJ-27
App 4 Cautions
AJ-41
App 5 Suppliers
AJ-55
App 6 Trademarks
AJ-56
Index
I-1
Backcover
I-23
Copyright

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