Physical Principles and Techniques of Protein Chemistry Part A, Part 1
Elsevier, Dec 2, 2012 - Science - 544 pages
Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation.
This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins’ dynamic properties and rate constants. Other chapters deal with the evaluation of proteins’ dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight.
This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.
What people are saying - Write a review
We haven't found any reviews in the usual places.
Chapter 2 XRay Methods
Chapter 3 Ultraviolet Absorption
Chapter 4 Fluorescence of Proteins
Chapter 5 Perturbation and Flow Techniques
Chapter 6 Dielectric Properties of Proteins I Dielectric Relaxation
Chapter 7 Dielectric Properties of Proteins II Electric Birefringence and Dichroism
absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefﬁcient concentration curve deﬁned denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction diffusion dipole moment Edelhoch effects electric birefringence electric ﬁeld electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor ﬁeld strength ﬁlm ﬁlters ﬁrst ﬂow ﬂuorescence fraction frequency gel ﬁltration groups intensity interactions ionic strength ions light macromolecules magniﬁcation measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic phenylalanine photomultiplier Phys plot polarization polymer protein quantum yield ratio reaction reﬂections relaxation residues ribonuclease rotation shown in Fig signiﬁcant solution solvent speciﬁc specimen spectra structure sufﬁciently technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone