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MATERIALS AND METHODS
THE PHYTOTOXINS OF CERATOCYSTIS
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absorption spectra acetic agar amino acids amino sugars anthrone antigenic antiserum aqueous assay biochemical browning reactions buffers carbohydrate cellulase Ceratocystis ulmi Chem chemical chromatography on Sepharose column compounds concentration crude toxin culture filtrate degradation described detected dialysed dilute Dimond disease symptoms diseased elms Dutch elm disease elm sprouts enzymatic ethanol experiments Feldman filtrate of Ceratocystis freeze-drying fungus Gagnon gel permeation chromatography hydrolysis incubated latter main fractions material method methylated product mixture monosaccharides ninhydrin normal obtained partial specific volume peak pectin pectinase peptide phytotoxic phytotoxic activity phytotoxin plant polysaccharide precipitate preparative centrifuging procedure pronase pronase-treated toxin protein proteolytic enzymes quantities reagent resistant samples sedimentation constant Sedimentation pattern separation Sephadex Sepharose Sepharose 4B solvent spores starting preparation structure studied substance Tchernoff thin-layer chromatography toxic toxin component toxin fractions toxin preparations ultracentrifuge ultraviolet absorption viscosity vivo vivotoxin wilt xylem xylem sap xylem vessels Zentmyer zijn