Pitfalls and Errors of HPLC in Pictures
The third edition of this popular problem-solving guide for this widely-used method includes eleven completely new examples and
several updated ones, adding up to 100 contributions about pitfalls and errors in HPLC. Each example is presented on a double page with the
text on the left-hand and a fi gure on the right-hand side, true to the motto 'a picture says more than a thousand words'. In addition, the
author presents essential fundamentals as well as helpful strategies, such as equipment tests or quality assurance processes.
New in this edition
* Variability of the standard deviation
* Infl uence of the acid type and concentration in the eluent
* Water as an unintentional additive in the mobile phase
* Inadequate purity of mobile phase water
* Incomplete degassing
* Inadequate stabilization of the extraction solvent
* Tailing of phosphate compounds in the presence of steel
* Different detection properties of diastereomers
* Detector overload in ELSD
* System suitability test
* From repeatability to reproducibility
A must-have resource for all users - showing how to use HPLC efficiently and obtain reliable results.
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25 cm Stationary 254 nm Reference accuracy accurate acetonitrile acid amount amylene analytical result anthracyclines autosampler baseline buffer pH calibration curve capillaries chiral Chromatographic Conditions Sample cm Stationary phase coefficient column compounds concentration control chart data points determination diastereomers diode array detector dissolved in mobile dwell volume eluent eluted enantiomers example extra-column volume flow rate gradient separations graph hexane HPLC identical impurities increase injection injector integration Ishikawa diagram laboratory LC GC loop lower methanol method mL min—1 Detector mL min—1 Temperature mobile phase necessary noise parameters peak area peak heights peak shape performed phosphate pm reversed phase pm silica possible precision problem procedure quantitative analysis reagent relative standard deviation resolution retention factor reversed phase C18 ruggedness sample preparation sample solution sample solvent signal small peak sodium solvent SOPs system peaks tailing theoretical plate theoretical plate number V.R. Meyer Validation vials water/acetonitrile water/methanol wavelength Zorbax