Placenta and Trophoblast: Methods and Protocols, Volume 2
Michael J. Soares, Joan S. Hunt
Springer Science & Business Media, Nov 1, 2005 - Medical - 476 pages
A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.
What people are saying - Write a review
We haven't found any reviews in the usual places.
Other editions - View all
acid transport activity amino acid analysis antibody antigens apoptotic approx assay BeWo Biol biopsies blood buffer cannula cDNA cell culture cell line cell suspension centrifuge column concentration containing culture medium cytokines cytotrophoblasts decidual differentiation dilution DMEM ELISA enzyme explants expression extravillous fatty acid fetal fetus filter flask flow cytometry forceps function gene HLA-G hormone human placental hypertension immune immunohistochemistry incubation injection Invitrogen isolated leukocytes macrophages maternal membrane Methods and Protocols mice Molecular monocytes mouse NK cells Note oocytes ovine oxygen PAGs pellet perfusion pipet placental placental bed plasma plasmid plate preeclampsia pregnancy prepared protein purified radiolabeled reagents receptor Reprod Resuspend room temperature RPMI samples sections serum Sigma slides solution spiral arteries staining sterile studies substrate supernatant syncytiotrophoblast tion tissue transfected transfer trophoblast trophoblast cells trophoblast invasion tube umbilical uptake uterine uteroplacental uterus vessel vials villous vitro wash